Background:

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.

Applications:

WB

Name of antibody:

Strep tag II

Immunogen:

Synthetic peptide: Strep tag II conjugated to KLH

Full name:

Strep tag II

WB Recommended dilution:

5000-20000