Background:

The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Applications:

WB, IF, IP

Name of antibody:

6×His Tag

Immunogen:

Synthetic peptide conjugated with KLH

Full name:

HHHHHH Tag

Synonyms:

6 His epitope tag

WB Predicted band size:

50 kDa

WB Positive control:

Fusion proteins containing the His Tag

WB Recommended dilution:

1000-5000

IF Positive control:

3T3 cells

IF Recommended dilution:

100-500

IP Recommended dilution:

100-200